验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, human heart tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14573-1-AP targets PPM1K in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag5660 Product name: Recombinant human PPM1K protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-372 aa of BC037552 Sequence: MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1K (PP2C domain containing) |
| 别名 | PP2C kappa, PP2Cm, PPM1K, PTMP, UG0882E07 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC037552 |
| 基因名称 | PPM1K |
| Gene ID (NCBI) | 152926 |
| RRID | AB_2252953 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N3J5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPM1K antibody 14573-1-AP | Download protocol |
| WB protocol for PPM1K antibody 14573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Glucose promotes cell growth by suppressing branched-chain amino acid degradation.
| ||
Nat Commun Branched-chain α-keto acids impair glucose-stimulated insulin secretion in pancreatic β-cells under diabetes by reactivating the LDHA-lactate axis. | ||
Free Radic Biol Med Impaired BCAA catabolism in macrophages exacerbates sepsis-induced lung injury by modulating mitochondrial AGE-RAGE and mtDNA release. | ||
Free Radic Biol Med Elevated branched-chain α-keto acids exacerbate macrophage oxidative stress and chronic inflammatory damage in type 2 diabetes mellitus. | ||
Biochem Pharmacol BCAA exaggerated acute and chronic ischemic heart disease through promotion of NLRP3 via Sirt1 | ||
Diabetes Obes Metab Dietary BCAA supplementation counteracts the beneficial effects of long-term aerobic exercise against obesity and associated metabolic disorders by suppressing FGF21 expression |
