验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16269-1-AP targets PPM1J in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9336 Product name: Recombinant human PPM1J protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-488 aa of BC011803 Sequence: DSRAIIVRNGEIIPMSREFTPETERQRLQLLGFLKPELLGSEFTHLEFPRRVLPKELGQRMLYRDQNMTGWAYKKIELEDLRFPLVCGEGKKARVMATIGVTRGLGDHSLKVCSSTLPIKPFLSCFPEVRVYDLTQYEHCPDDVLVLGTDGLWDVTTDCEVAATVDRVLSAYEPNDHSRYTALAQALVLGARGTPRDRGWRLPNNKLGSGDDISVFVIPLGGPGSYS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1J (PP2C domain containing) |
| 别名 | PPP2CZ, PP2C-zeta, PP2Czeta, PP2CZ, PP2C zeta |
| 计算分子量 | 505 aa, 54 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC011803 |
| 基因名称 | PPM1J |
| Gene ID (NCBI) | 333926 |
| RRID | AB_3669235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5JR12 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPM1J also known as PPP2CZ, PP2C-zeta, belongs to the PP2C family. It has a unique N-terminal region and a proline-rich domain between conserved motifs II and III.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPM1J antibody 16269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
