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  • Featured Product
  • KD/KO Validated

PPM1F Polyclonal antibody

PPM1F Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17020-1-AP

Print datasheet

Synonyms

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

验证数据展示

  • WB analysis of MCF-7 using 17020-1-AP

    WB analysis of MCF-7 using 17020-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 17020-1-AP

    IP experiment of MCF-7 using 17020-1-AP

    IP result of anti-PPM1F (IP:17020-1-AP, 4ug; Detection:17020-1-AP 1:700) with MCF-7 cells lysate 2800ug.

  • IF Staining of HepG2 using 17020-1-AP

    IF Staining of HepG2 using 17020-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17020-1-AP (PPM1F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 17020-1-AP

    WB analysis using 17020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17020-1-AP (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, L02 cells, HepG2 cells, human liver tissue, K-562 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17020-1-AP targets PPM1F in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen PPM1F fusion protein Ag10186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1F (PP2C domain containing)
Synonyms CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC071989
Gene Symbol PPM1F
Gene ID (NCBI) 9647
RRIDAB_2284338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49593
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

实验方案

Product Specific Protocols
WB protocol for PPM1F antibody 17020-1-APDownload protocol
IF protocol for PPM1F antibody 17020-1-APDownload protocol
IP protocol for PPM1F antibody 17020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Biol

PPM1F controls integrin activity via a conserved phospho-switch.

Authors - Tanja M Grimm
  • KO Validated
View Article