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验证数据展示

  • WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Sample test of MP51322-1

    Sample test of MP51322-1

    Sample test of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS.

  • Sample test of MP51322-2

    Sample test of MP51322-2

    Sample test of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS.

  • Sample test of MP51322-3

    Sample test of MP51322-3

    Sample test of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS.

  • Sample test of MP51322-4

    Sample test of MP51322-4

    Sample test of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS.

  • IF Staining of A431 using 67647-1-Ig (same clone as 67647-1-PBS)

    IF Staining of A431 using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

PPM1B Monoclonal antibody, PBS Only (Capture)

PPM1B Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 67647-1-PBS

产品说明书

CloneNo. 2C7H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

别名

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67647-1-PBS targets PPM1B as part of a matched antibody pair:

MP51322-1: 67647-1-PBS capture and 67647-2-PBS detection (validated in Cytometric bead array)

MP51322-2: 67647-1-PBS capture and 67647-3-PBS detection (validated in Cytometric bead array)

MP51322-3: 67647-1-PBS capture and 67647-4-PBS detection (validated in Cytometric bead array)

MP51322-4: 67647-1-PBS capture and 67647-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 PPM1B fusion protein Ag3871 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
别名 2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta
计算分子量 479 aa, 53 kDa
观测分子量 36, 53 kDa
GenBank蛋白编号BC012002
基因名称 PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75688
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.