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PPIL4 Polyclonal antibody

PPIL4 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12538-1-AP

Print datasheet

Synonyms

Cyclophilin like protein PPIL4, HDCME13P, PPIase, PPIL4, Rotamase PPIL4

验证数据展示

  • WB analysis using 12538-1-AP

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12538-1-AP

    IP experiment of HeLa using 12538-1-AP

    IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • WB analysis using 12538-1-AP

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12538-1-AP

    IF Staining of HeLa using 12538-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12538-1-AP (PPIL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12538-1-AP targets PPIL4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPIL4 fusion protein Ag3249 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase (cyclophilin)-like 4
Synonyms Cyclophilin like protein PPIL4, HDCME13P, PPIase, PPIL4, Rotamase PPIL4
Calculated Molecular Weight 492 aa, 57 kDa
Observed Molecular Weight 58-65 kDa
GenBank Accession NumberBC016984
Gene Symbol PPIL4
Gene ID (NCBI) 85313
RRIDAB_10644441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL4 antibody 12538-1-APDownload protocol
IF protocol for PPIL4 antibody 12538-1-APDownload protocol
IP protocol for PPIL4 antibody 12538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.

Authors - Wenbo Liu
View Article