• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12538-1-AP

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12538-1-AP

    IP experiment of HeLa using 12538-1-AP

    IP result of anti-PPIL4 (IP:12538-1-AP, 4ug; Detection:12538-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • WB analysis using 12538-1-AP

    WB analysis using 12538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12538-1-AP (PPIL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12538-1-AP

    IF Staining of HeLa using 12538-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPIL4 antibody (12538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PPIL4 Polyclonal antibody

PPIL4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12538-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Cyclophilin like protein PPIL4, Cyclophilin-like protein PPIL4, EC:5.2.1.8, HDCME13P, Peptidyl-prolyl cis-trans isomerase-like 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12538-1-AP targets PPIL4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPIL4 fusion protein Ag3249 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase (cyclophilin)-like 4
别名 Cyclophilin like protein PPIL4, Cyclophilin-like protein PPIL4, EC:5.2.1.8, HDCME13P, Peptidyl-prolyl cis-trans isomerase-like 4
计算分子量 492 aa, 57 kDa
观测分子量 58-65 kDa
GenBank蛋白编号BC016984
基因名称 PPIL4
Gene ID (NCBI) 85313
RRIDAB_10644441
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WUA2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL4 belongs to the cyclophilin subgroup of the peptidyl-prolyl cis-trans isomerase (PPIase) protein family (PPIs). It contains an RNA recognition motif and a PPIase-like domain. PPIL4, as an IA susceptibility gene, is a novel and key regulatory molecule in Wnt signaling and vertebrate cerebrovascular development (PMID: 34887573).

实验方案

Product Specific Protocols
WB protocol for PPIL4 antibody 12538-1-APDownload protocol
IF protocol for PPIL4 antibody 12538-1-APDownload protocol
IP protocol for PPIL4 antibody 12538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.

Authors - Wenbo Liu
View Article