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验证数据展示

  • IP experiment of HEK-293 using 12590-2-AP

    IP experiment of HEK-293 using 12590-2-AP

    IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug.

  • WB analysis of human kidney using 12590-2-AP

    WB analysis of human kidney using 12590-2-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

PPIL2 Polyclonal antibody

PPIL2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12590-2-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, HEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12590-2-AP targets PPIL2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag3296

Product name: Recombinant human PPIL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-520 aa of BC028385

Sequence: LAATMKAPEKKKVDKLNAAHYSTGKVSASFTSTAMVPETTHEAAAIDEDVLRYQFVKKKGYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANSGPNSNRSQFFITFRSCAYLDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVFVDPYEEADAQIAQERKTQLKIAPETKVKSSQPQAGSQGPQTFRQGVGKYINPAATKRAAEEEPSTSATVPMSKKKPSRGFGDFSSW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase (cyclophilin)-like 2
别名 CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2
计算分子量 520 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC028385
基因名称 PPIL2
Gene ID (NCBI) 23759
RRIDAB_2169606
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13356
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL2 antibody 12590-2-APDownload protocol
IP protocol for PPIL2 antibody 12590-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Cardiovasc Med

Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.

Authors - Yan Xu
View Article