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验证数据展示

  • WB analysis of NIH/3T3 using 15144-1-AP

    WB analysis of NIH/3T3 using 15144-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15144-1-AP

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPIL1 antibody included
  • IHC staining of human colon cancer using 15144-1-AP

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPIL1 antibody included
  • IP experiment of NIH/3T3 using 15144-1-AP

    IP experiment of NIH/3T3 using 15144-1-AP

    IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug.

  • IF Staining of NIH/3T3 using 15144-1-AP

    IF Staining of NIH/3T3 using 15144-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PPIL1 Polyclonal antibody

PPIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15144-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CGI 124, CGI-124, CYPL1, EC:5.2.1.8, hCyPX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A375 cells, HeLa cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PPIL1 Ready-To-Use IHC Kit. PPIL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

15144-1-AP targets PPIL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPIL1 fusion protein Ag7436 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase (cyclophilin)-like 1
别名 CGI 124, CGI-124, CYPL1, EC:5.2.1.8, hCyPX
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC003048
基因名称 PPIL1
Gene ID (NCBI) 51645
RRIDAB_2169603
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3C6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL1 (Peptidyl-prolyl cis-trans isomerase-like 1) is up-regulated in human colon cancer cells and an siRNA-mediated knockdown of PPIL1 resulted in a reduced number of viable cell (PMID: 20368803). Expression of PPIL1 was shown to be ubiquitous across all adult tissues (with highest expression in the adrenal gland, testis and heart). PPIL1 is also known to participate in the spliceosome; a complex and dynamic protein and RNA complex responsible for splicing introns from pre-mRNA (PMID: 33220177, 8978786).

实验方案

Product Specific Protocols
WB protocol for PPIL1 antibody 15144-1-APDownload protocol
IHC protocol for PPIL1 antibody 15144-1-APDownload protocol
IF protocol for PPIL1 antibody 15144-1-APDownload protocol
IP protocol for PPIL1 antibody 15144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Neuron

Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly.

Authors - Guoliang Chai
View Article