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PPIL1 Polyclonal antibody

PPIL1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15144-1-AP

Print datasheet

Synonyms

CGI 124, CYPL1, hCyPX, PPIase, PPIL1, Rotamase PPIL1, UNQ2425/PRO4984

验证数据展示

  • WB analysis of NIH/3T3 using 15144-1-AP

    WB analysis of NIH/3T3 using 15144-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15144-1-AP

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with PPIL1 antibody included
  • IHC staining of human colon cancer using 15144-1-AP

    IHC staining of human colon cancer using 15144-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with PPIL1 antibody included
  • IP experiment of NIH/3T3 using 15144-1-AP

    IP experiment of NIH/3T3 using 15144-1-AP

    IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug.

  • IF Staining of NIH/3T3 using 15144-1-AP

    IF Staining of NIH/3T3 using 15144-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inNIH/3T3 cells, A375 cells, HeLa cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PPIL1 Ready-To-Use IHC Kit. PPIL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

15144-1-AP targets PPIL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PPIL1 fusion protein Ag7436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidylprolyl isomerase (cyclophilin)-like 1
Synonyms CGI 124, CYPL1, hCyPX, PPIase, PPIL1, Rotamase PPIL1, UNQ2425/PRO4984
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC003048
Gene Symbol PPIL1
Gene ID (NCBI) 51645
RRIDAB_2169603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPIL1 antibody 15144-1-APDownload protocol
IHC protocol for PPIL1 antibody 15144-1-APDownload protocol
IF protocol for PPIL1 antibody 15144-1-APDownload protocol
IP protocol for PPIL1 antibody 15144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Neuron

Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly.

Authors - Guoliang Chai
View Article