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验证数据展示

  • WB analysis of U2OS using 11651-1-AP

    WB analysis of U2OS using 11651-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 11651-1-AP (PPIH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 11651-1-AP

    WB analysis using 11651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11651-1-AP (PPIH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11651-1-AP

    IHC staining of human colon cancer using 11651-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11651-1-AP (PPIH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 11651-1-AP

    IHC staining of human colon cancer using 11651-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11651-1-AP (PPIH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 11651-1-AP

    IF Staining of PC-3 using 11651-1-AP

    Immunofluorescent analysis of PC-3 cells using 11651-1-AP (PPIH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PPIH Polyclonal antibody

PPIH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11651-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

PPIase H, Peptidyl-prolyl cis-trans isomerase H, EC:5.2.1.8, CypH, CYP20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, K-562 cells, PC-3 cells, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

11651-1-AP targets PPIH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 PPIH fusion protein Ag2253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidylprolyl isomerase H (cyclophilin H)
别名 PPIase H, Peptidyl-prolyl cis-trans isomerase H, EC:5.2.1.8, CypH, CYP20
计算分子量 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC003412
基因名称 PPIH
Gene ID (NCBI) 10465
RRIDAB_2284306
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43447
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPIH(Peptidyl-prolyl cis-trans isomerase H) is also named as CYP20, CYPH and belongs to cyclophilin-type PPIase family, PPIase H subfamily.It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may act as a chaperone(PMID:12875835).

实验方案

Product Specific Protocols
WB protocol for PPIH antibody 11651-1-APDownload protocol
IHC protocol for PPIH antibody 11651-1-APDownload protocol
IF protocol for PPIH antibody 11651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Theranostics

Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Authors - Yinan Chen
View Article