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验证数据展示

  • WB analysis using 84115-1-RR (same clone as 84115-1-PBS)

    WB analysis using 84115-1-RR (same clone as 84115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84115-1-RR (PPID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 84115-1-RR (same clone as 84115-1-PBS)

    FC experiment of A431 using 84115-1-RR (same clone as 84115-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PPID Recombinant Antibody (84115-1-RR, Clone:241361B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84115-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84115-1-PBS

    Affinity and Kinetic Characterization of 84115-1-PBS

    Biolayer interferometry (BLl) kinetic assay of 84115-1-PBS against Human PPID was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84115-1-RR

    Affinity and Kinetic Characterization of 84115-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84115-1-RR against Human PPID were performed. The affinity constant is 0.313 nM.

PPID Recombinant monoclonal antibody, PBS Only

PPID Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84115-1-PBS

产品说明书

CloneNo. 241361B2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

241361B2, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin 40, Cyclophilin D, Cyclophilin-40

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPID Ready-To-Use IHC Kit. PPID primary antibody included.

产品信息

84115-1-PBS targets PPID in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3409

Product name: Recombinant human PPID protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 57-370 aa of BC030707

Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidylprolyl isomerase D
别名 241361B2, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin 40, Cyclophilin D, Cyclophilin-40
计算分子量 370 aa, 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC030707
基因名称 PPID
Gene ID (NCBI) 5481
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ08752
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

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