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验证数据展示

  • WB analysis using 19912-1-AP (same clone as 19912-1-PBS)

    WB analysis using 19912-1-AP (same clone as 19912-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 19912-1-AP (same clone as 19912-1-PBS)

    IHC staining of human colon cancer using 19912-1-AP (same clone as 19912-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19912-1-AP (C20orf149 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 19912-1-AP (same clone as 19912-1-PBS)

    IF Staining of U2OS using 19912-1-AP (same clone as 19912-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

PPDPF Polyclonal antibody, PBS Only

PPDPF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19912-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C20orf149, EXPDF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19912-1-PBS targets PPDPF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13740

Product name: Recombinant human C20orf149 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-114 aa of BC002531

Sequence: MAAIPSSGSLVATHDYYRRRLGSTSSNSSCSSTECPGEAIPHPPGLPKADPGHWWASFFFGKSTLPFMATVLESAEHSEPPQASSSMTACGLARDAPRKQPGGQSSTASAGPPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 149
别名 C20orf149, EXPDF
计算分子量 114 aa, 12 kDa
观测分子量 10-12 kDa
GenBank蛋白编号BC002531
基因名称 PPDPF
Gene ID (NCBI) 79144
RRIDAB_10642438
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3Y8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.

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