验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human placenta tissue, HL-60 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16270-1-AP targets PPCDC in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PPCDC fusion protein Ag9337 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphopantothenoylcysteine decarboxylase |
| 别名 | CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154 |
| 计算分子量 | 204 aa, 22 kDa |
| 观测分子量 | 22-25 kDa, 14 kDa |
| GenBank蛋白编号 | BC014409 |
| 基因名称 | PPCDC |
| Gene ID (NCBI) | 60490 |
| RRID | AB_2299841 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96CD2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPCDC antibody 16270-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
