- Featured Product
- KD/KO Validated
PPAT Polyclonal antibody
PPAT Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 20 publications below |
产品信息
15401-1-AP targets PPAT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PPAT fusion protein Ag7618 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | phosphoribosyl pyrophosphate amidotransferase |
| Synonyms | Amidophosphoribosyltransferase, ATASE, GPAT, PPAT, PRAT |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 50-56 kDa |
| GenBank Accession Number | BC004200 |
| Gene Symbol | PPAT |
| Gene ID (NCBI) | 5471 |
| RRID | AB_2166532 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06203 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPAT antibody 15401-1-AP | Download protocol |
| IP protocol for PPAT antibody 15401-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. | ||
Cancer Cell Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. | ||
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Nat Commun A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer. | ||
EMBO J UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.
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