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验证数据展示

  • WB analysis using 67821-1-Ig

    WB analysis using 67821-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 67821-1-Ig

    WB analysis of SH-SY5Y using 67821-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PPARGC1B Monoclonal antibody

PPARGC1B Monoclonal Antibody for WB, ELISA
Cat No. 67821-1-Ig

产品说明书

CloneNo. 2D2E8
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig, rat

应用

WB, ELISA

别名

ERRL1, PERC, PGC 1 beta, PGC 1(beta), PGC1, PGC1B, PPAR gamma coactivator 1 beta, PPARGC 1 beta, PPARGC1, PPARGC1B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, SH-SY5Y cells, pig brain tissue, rat brain tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67821-1-Ig targets PPARGC1B in WB, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, pig, rat
文献引用反应性mouse, rat
免疫原

CatNo: Ag28861

Product name: Recombinant human PPARGC1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC132971

Sequence: MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxisome proliferator-activated receptor gamma, coactivator 1 beta
别名 ERRL1, PERC, PGC 1 beta, PGC 1(beta), PGC1, PGC1B, PPAR gamma coactivator 1 beta, PPARGC 1 beta, PPARGC1, PPARGC1B
计算分子量 1023 aa, 113 kDa
观测分子量150 kDa
GenBank蛋白编号BC132971
基因名称 PPARGC1B
Gene ID (NCBI) 133522
RRIDAB_2918584
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ86YN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPARGC1B antibody 67821-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Chem Biol Interact

N-acetylcysteine delayed cadmium-induced chronic kidney injury by activating the sirtuin 1-P53 signaling pathway

Authors - Wenxuan Dong
View Article
mouseWB

Sci Rep

Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice

Authors - Yusuke Komiya
View Article