验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22378-1-AP targets PPARGC1B in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, zebrafish, goat, fish, megalobrama amblycephala |
| 免疫原 |
CatNo: Ag17566 Product name: Recombinant human PPARGC1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC132971 Sequence: MAGNDCGALLDEELSSFFLNYLADTQGGGSGEEQLYADFPELDLSQLDASDFDSATCFGELQWCPENSETEPNQYSPDDSELFQIDSENEALLAELTKTLDDIPEDDVGLAAFPALDGGDALSCTSASPAPSSAPPSPAPEKPSAPAPEVDELSLLQKLLLATSYPTSSSDTQKEGTAWRQAGLRSKSQRPCVKADSTQDKKAPMMQSQSRSCTELHKHLTSAQCCLQDRGLQPPCLQSPRLPAKEDKEPGEDCPSPQPAPASPRDSLALGRADPGAPVSQEDMQAMVQLIRYMHTYCLPQRKLPPQTPEPLPKACSNPSQQVRSRPWSRHHSKASWAEFSILRELLAQD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxisome proliferator-activated receptor gamma, coactivator 1 beta |
| 别名 | ERRL1, PERC, Peroxisome proliferator activated receptor gamma coactivator 1 beta, Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC1 |
| 计算分子量 | 1023 aa, 113 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | BC132971 |
| 基因名称 | PPARGC1B |
| Gene ID (NCBI) | 133522 |
| RRID | AB_2879093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86YN6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPARGC1B (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Beta) is a transcriptional coactivator that plays a key role in the regulation of cellular energy metabolism, particularly in tissues with high metabolic demands such as the heart, skeletal muscle, liver, and brain. Encoded by the PPARGC1B gene in humans, it interacts with a variety of nuclear receptors and transcription factors, including PPARs, ERRs, and NRF1, to promote the expression of genes involved in mitochondrial biogenesis, oxidative phosphorylation, fatty acid oxidation, and glucose homeostasis. Unlike its close paralog PGC-1α, PGC-1β is less responsive to physiological stimuli like cold exposure or exercise but is more constitutively expressed and tightly linked to lipid metabolism and reactive oxygen species (ROS) detoxification.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| WB protocol for PPARGC1B antibody 22378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Redox Biol miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation | ||
Br J Pharmacol Andrographolide prevents bone loss via targeting ERRalpha-regulated metabolic adaption of osteoclastogenesis. | ||
EMBO Rep Mitophagy receptor FUNDC1 is regulated by PGC-1α/NRF1 to fine tune mitochondrial homeostasis.
| ||
J Anim Sci Biotechnol miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta. | ||
Nutrients Novel Choline-Deficient and 0.1%-Methionine-Added High-Fat Diet Induces Burned-Out Metabolic-Dysfunction-Associated Steatohepatitis with Inflammation by Rapid Immune Cell Infiltration on Male Mice | ||
FASEB J Hypolipidemic effect of oleanolic acid is mediated by the miR-98-5p/PGC-1β axis in high-fat diet-induced hyperlipidemic mice.
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