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Host
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验证数据展示

  • WB analysis using 85445-3-RR (same clone as 85445-3-PBS)

    WB analysis using 85445-3-RR (same clone as 85445-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85445-3-RR (PPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85445-3-RR

    Affinity and Kinetic Characterization of 85445-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85445-3-RR against Human PPA1 were performed. The affinity constant is 4.07 nM.

PPA1 Recombinant antibody, PBS Only

PPA1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85445-3-PBS

产品说明书

CloneNo. 242963D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

242963D1, EC:3.6.1.1, Inorganic pyrophosphatase, IOPPP, PP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85445-3-PBS targets PPA1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPA1 fusion protein Ag6969 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyrophosphatase (inorganic) 1
别名 242963D1, EC:3.6.1.1, Inorganic pyrophosphatase, IOPPP, PP
计算分子量 33 kDa
观测分子量 33 kDa
GenBank蛋白编号BC001022
基因名称 PPA1
Gene ID (NCBI) 5464
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15181
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).