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验证数据展示

  • WB analysis of SH-SY5Y using 18999-1-AP

    WB analysis of SH-SY5Y using 18999-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 18999-1-AP

    IHC staining of human brain using 18999-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 18999-1-AP

    IHC staining of human brain using 18999-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18999-1-AP (POU3F3-Specific antibody) at dilution of 1:100 (under 40x lens).

POU3F3-Specific Polyclonal antibody

POU3F3-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18999-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, human brain tissue, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18999-1-AP targets POU3F3-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POU class 3 homeobox 3
别名 Brain 1, Brn 1, BRN1, Oct 8, Octamer binding protein 8, OTF 8, OTF8, POU class 3 homeobox 3, POU3F3
计算分子量 50 kDa
观测分子量 66 kDa
GenBank蛋白编号NM_006236
基因名称 POU3F3
Gene ID (NCBI) 5455
RRIDAB_2284130
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20264
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OTF8, also named as POU3F3 and BRN1, belongs to the POU transcription factor family and Class-3 subfamily. The antibody is specific to OTF8.

实验方案

Product Specific Protocols
WB protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
IHC protocol for POU3F3-Specific antibody 18999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dev Cell

AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.

Authors - Alexander G Marneros
View Article
humanWB

Int Immunopharmacol

Iguratimod-encapsulating PLGA-NPs induce human multiple myeloma cell death via reactive oxygen species and Caspase-dependent signalling.

Authors - Faming Wang
View Article
humanIHC

Histochem Cell Biol

Connecting tubules develop from the tip of the ureteric bud in the human kidney.

Authors - Beatrix Sarkany
View Article