验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, human brain tissue, human spleen tissue, mouse kidney tissue, Raji cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
18996-1-AP targets OCT2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | POU class 2 homeobox 2 |
| 别名 | OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2 |
| 计算分子量 | 62 kDa |
| 观测分子量 | 52-66 kDa |
| GenBank蛋白编号 | NM_002698 |
| 基因名称 | POU2F2 |
| Gene ID (NCBI) | 5452 |
| RRID | AB_10596624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OCT2-Specific antibody 18996-1-AP | Download protocol |
| IHC protocol for OCT2-Specific antibody 18996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy | ||
Mol Ther miR-3687 Overexpression Promotes Bladder Cancer Cell Growth by Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription. | ||
Int J Oncol Receptor for activated C kinase 1 promotes cervical cancer lymph node metastasis via the glycolysis‑dependent AKT/mTOR signaling. | ||
Biochem Biophys Res Commun CBX7 represses the POU2F2 to inhibit the PD-L1 expression and regulate the immune response in bladder cancer.
| ||
Toxicol In Vitro Comparisons of segment-specific toxicity of platinum-based agents and cadmium using S1, S2, and S3 cells derived from mouse kidney proximal tubules. | ||
