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OCT2-Specific Polyclonal antibody

OCT2-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18996-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue, human brain tissue, human spleen tissue, mouse kidney tissue, Raji cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18996-1-AP targets OCT2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POU class 2 homeobox 2
别名 OCT2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Oct-2, Octamer-binding protein 2
计算分子量 62 kDa
观测分子量52-66 kDa
GenBank蛋白编号NM_002698
基因名称 POU2F2
Gene ID (NCBI) 5452
RRIDAB_10596624
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OCT2, also named as POU2F2 and OTF2, belongs to the POU transcription factor family and Class-2 subfamily. OCT2 is a transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). OCT2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. The antibody is specific to OCT2. OCT2 can be detected 52-66 kDa (PMID: 15377492, PMID: 34010659).

实验方案

Product Specific Protocols
WB protocol for OCT2-Specific antibody 18996-1-APDownload protocol
IHC protocol for OCT2-Specific antibody 18996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy

Authors - Chen Yang
humanWB

Mol Ther

miR-3687 Overexpression Promotes Bladder Cancer Cell Growth by Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription.

Authors - Qipeng Xie
mouse,humanWB,IHC

Int J Oncol

Receptor for activated C kinase 1 promotes cervical cancer lymph node metastasis via the glycolysis‑dependent AKT/mTOR signaling.

Authors - Lixiu Xu
humanWB

Biochem Biophys Res Commun

CBX7 represses the POU2F2 to inhibit the PD-L1 expression and regulate the immune response in bladder cancer.

Authors - Weigang Ren
  • KD Validated
WB

Toxicol In Vitro

Comparisons of segment-specific toxicity of platinum-based agents and cadmium using S1, S2, and S3 cells derived from mouse kidney proximal tubules.

Authors - Hitomi Fujishiro
mouseWB

Age (Dordr)

Aging increases the susceptibility of cisplatin-induced nephrotoxicity.

Authors - Jiagen Wen
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