验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, mouse ovary tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| ChIP | See 1 publications below |
产品信息
10867-2-AP targets OCT2 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | OCT2 fusion protein Ag0955 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | POU class 2 homeobox 2 |
| 别名 | Oct 2, OCT2, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC006101 |
| 基因名称 | POU2F2 |
| Gene ID (NCBI) | 5452 |
| RRID | AB_2167080 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OCT2 antibody 10867-2-AP | Download protocol |
| IHC protocol for OCT2 antibody 10867-2-AP | Download protocol |
| IP protocol for OCT2 antibody 10867-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Renoprotective Effects of Brown-Strain Flammulina velutipes Singer in Chronic Kidney Disease-Induced Mice Through Modulation of Oxidative Stress and Inflammation and Regulation of Renal Transporters |
