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验证数据展示

  • WB analysis using 20613-1-AP

    WB analysis using 20613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20613-1-AP (PORCN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 20613-1-AP

    IHC staining of rat kidney using 20613-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20613-1-AP

    IHC staining of mouse brain using 20613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PORCN Polyclonal antibody

PORCN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20613-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DHOF, EC:2.3.1.250, FODH, MG61, por

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20613-1-AP targets PORCN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PORCN fusion protein Ag14664 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 porcupine homolog (Drosophila)
别名 DHOF, EC:2.3.1.250, FODH, MG61, por
计算分子量 461 aa, 52 kDa
观测分子量43~50 kDa
GenBank蛋白编号BC019080
基因名称 PORCN
Gene ID (NCBI) 64840
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PORCN is one of several related membrane-bound O-acyltransferase (MBOAT) enzymes encoding a 461 amino acid-sized (52 kDa) protein named porcupine located in the endoplasmic reticulum and known to be involved in the processing, secretion, and signaling of Wnt proteins (PMID: 17546031). Overexpression of PORCN could promote the proliferation and migration abilities of HCC cells both in vitro and in vivo (PMID: 37588740, 23188502).

实验方案

Product Specific Protocols
WB protocol for PORCN antibody 20613-1-APDownload protocol
IHC protocol for PORCN antibody 20613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols