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验证数据展示

  • WB analysis using 20613-1-AP

    WB analysis using 20613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20613-1-AP (PORCN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat kidney using 20613-1-AP

    IHC staining of rat kidney using 20613-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20613-1-AP

    IHC staining of mouse brain using 20613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PORCN Polyclonal antibody

PORCN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20613-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DHOF, EC:2.3.1.250, FODH, MG61, por

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inmouse brain tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20613-1-AP targets PORCN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14664

Product name: Recombinant human PORCN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 258-348 aa of BC019080

Sequence: EATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 porcupine homolog (Drosophila)
别名 DHOF, EC:2.3.1.250, FODH, MG61, por
计算分子量 461 aa, 52 kDa
观测分子量43~50 kDa
GenBank蛋白编号BC019080
基因名称 PORCN
Gene ID (NCBI) 64840
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PORCN is one of several related membrane-bound O-acyltransferase (MBOAT) enzymes encoding a 461 amino acid-sized (52 kDa) protein named porcupine located in the endoplasmic reticulum and known to be involved in the processing, secretion, and signaling of Wnt proteins (PMID: 17546031). Overexpression of PORCN could promote the proliferation and migration abilities of HCC cells both in vitro and in vivo (PMID: 37588740, 23188502).

实验方案

Product Specific Protocols
IHC protocol for PORCN antibody 20613-1-APDownload protocol
WB protocol for PORCN antibody 20613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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