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验证数据展示

  • WB analysis of HepG2 using 15273-1-AP

    WB analysis of HepG2 using 15273-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15273-1-AP (POP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15273-1-AP

    IF Staining of HepG2 using 15273-1-AP

    Immunofluorescent analysis of HepG2 cells using 15273-1-AP (POP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POP4 Polyclonal antibody

POP4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15273-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RPP29, POP 4, hPOP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7387

Product name: Recombinant human POP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-220 aa of BC004438

Sequence: MKSVIYHALSQKEANDSDVQPSGAQRAEAFVRAFLKRSTPRMSPQAREDQLQRKAVVLEYFTRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLHELWKQYIRDLCSGLKPDTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae)
别名 RPP29, POP 4, hPOP4
计算分子量 25 kDa
观测分子量 29 kDa
GenBank蛋白编号BC004438
基因名称 POP4
Gene ID (NCBI) 10775
RRIDAB_2284033
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95707
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POP4 antibody 15273-1-APDownload protocol
IF protocol for POP4 antibody 15273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols