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验证数据展示

  • WB analysis using 22102-1-AP

    WB analysis using 22102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22102-1-AP (POMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 22102-1-AP

    IHC staining of human pituitary using 22102-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 22102-1-AP

    IF Staining of HeLa using 22102-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (22102-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

POMC Polyclonal antibody

POMC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22102-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CLIP, Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse pituitary gland tissue, Transfected HEK-293 cells
Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22102-1-AP targets POMC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 POMC fusion protein Ag17311 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proopiomelanocortin
别名 CLIP, Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha
计算分子量 267 aa, 29 kDa
观测分子量 35 kDa
GenBank蛋白编号BC065832
基因名称 POMC
Gene ID (NCBI) 5443
RRIDAB_3085685
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP01189
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

实验方案

Product Specific Protocols
WB protocol for POMC antibody 22102-1-APDownload protocol
IHC protocol for POMC antibody 22102-1-APDownload protocol
IF protocol for POMC antibody 22102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols