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验证数据展示

  • WB analysis using 15645-1-AP (same clone as 15645-1-PBS)

    WB analysis using 15645-1-AP (same clone as 15645-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 15645-1-AP (same clone as 15645-1-PBS)

    IF Staining of mouse brain using 15645-1-AP (same clone as 15645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

    IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 15645-1-AP (same clone as 15645-1-PBS)

    IF Staining of HCT 116 using 15645-1-AP (same clone as 15645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using POM121 antibody (15645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

POM121 Polyclonal antibody, PBS Only

POM121 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 15645-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15645-1-PBS targets POM121 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8177

Product name: Recombinant human POM121 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 635-984 aa of BC008794

Sequence: SQSLHTAVPTATSSSAADFSGFGSTLATSAPATSSQPTLTFSNTSTPTFNIPFGSSAKSPLPSYPGANPQPAFGAAEGQPPGAAKPALAPSFGSSFTFGNSAAPAAAPTPAPPSMIKVVPAYVPTPIHPIFGGATHSAFGLKATASAFGAPASSQPAFGGSTAVFFGAATSSGFGATTQTASSGSSSSVFGSTTPSPFTFGGSAAPAGSGSFGINVATPGSSTTTGAFSFGAGQSGSTATSTPFAGGLGQNALGTTGQSTPFAFNVSSTTESKPVFGGTATPTFGLNTPAPGVGTSGSSLSFGASSAPAQGFVGVAPFGSAALSFSIGAGSKTPGARQRLQARRQHTRKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 POM121 membrane glycoprotein (rat)
别名 POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121
计算分子量 984 aa, 99 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC008794
基因名称 POM121
Gene ID (NCBI) 9883
RRIDAB_2878160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HA1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

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