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验证数据展示

  • IF Staining of HepG2 using 11084-1-AP

    IF Staining of HepG2 using 11084-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR3K antibody (11084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 11084-1-AP

    IHC staining of mouse testis using 11084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11084-1-AP (POLR3K antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POLR3K Polyclonal antibody

POLR3K Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11084-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

HsC11p, hRPC11, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerase III subunit K, C11 RNP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11084-1-AP targets POLR3K in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1564

Product name: Recombinant human POLR3K protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-108 aa of BC011932

Sequence: MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
别名 HsC11p, hRPC11, DNA-directed RNA polymerase III subunit RPC10, DNA-directed RNA polymerase III subunit K, C11 RNP3
计算分子量 12 kDa
GenBank蛋白编号BC011932
基因名称 POLR3K
Gene ID (NCBI) 51728
RRIDAB_2165725
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Y1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for POLR3K antibody 11084-1-APDownload protocol
IHC protocol for POLR3K antibody 11084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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