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验证数据展示

  • WB analysis of human brain using 16044-1-AP

    WB analysis of human brain using 16044-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16044-1-AP

    IF Staining of HepG2 using 16044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16044-1-AP (POLR3F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

POLR3F Polyclonal antibody

POLR3F Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16044-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, BxPC-3 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16044-1-AP targets POLR3F in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8935

Product name: Recombinant human POLR3F protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC012588

Sequence: MAEVKVKVQPPDADPVEIENRIIELCHQFPHGITDQVIQNEMPHIEAQQRAVAINRLLSMGQLDLLRSNTGLLYRIKDSQNAGKMKGSDNQEKLVYQIIEDAGNKGIWSRDIRYKSNLPLTEINKILKNLESKKLIKAVKSVAASKKKVYMLYNLQPDRSVTGGAWYSDQDFESEFVEVLNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVELSMEDIETILNTLIYDGKVEMTIIAAKEGTVGSVDGHMKLYRAVNPIIPPTGLVRAPCGLCPVFDDCHEGGEISPSNCIYMTEWLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
别名 RPC6, RPC39, DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F
计算分子量 316 aa, 36 kDa
观测分子量 36-39 kDa
GenBank蛋白编号BC012588
基因名称 POLR3F
Gene ID (NCBI) 10621
RRIDAB_2165707
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1D9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for POLR3F antibody 16044-1-APDownload protocol
WB protocol for POLR3F antibody 16044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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