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POLR3F Polyclonal antibody

POLR3F Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16044-1-AP

Print datasheet

Synonyms

POLR3F, RNA polymerase III subunit C6, RPC39, RPC6

验证数据展示

  • WB analysis of human brain using 16044-1-AP

    WB analysis of human brain using 16044-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16044-1-AP (POLR3F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16044-1-AP

    IF Staining of HepG2 using 16044-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16044-1-AP (POLR3F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, BxPC-3 cells, HeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16044-1-AP targets POLR3F in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen POLR3F fusion protein Ag8935 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
Synonyms POLR3F, RNA polymerase III subunit C6, RPC39, RPC6
Calculated Molecular Weight 316 aa, 36 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC012588
Gene Symbol POLR3F
Gene ID (NCBI) 10621
RRIDAB_2165707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1D9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLR3F antibody 16044-1-APDownload protocol
IF protocol for POLR3F antibody 16044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols