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验证数据展示

  • WB analysis using 84367-6-RR

    WB analysis using 84367-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84367-6-RR (POLR3C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84367-6-RR

    IF Staining of HeLa using 84367-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLR3C antibody (84367-6-RR, Clone: 241402A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84367-6-RR

    Affinity and Kinetic Characterization of 84367-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84367-6-RR against Human POLR3C were performed. The affinity constant is below 1 pM.

POLR3C Recombinant antibody

POLR3C Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84367-6-RR

产品说明书

CloneNo. 241402A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

241402A9, DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, RPC3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84367-6-RR targets POLR3C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 POLR3C fusion protein Ag27311 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide C (62kD)
别名 241402A9, DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, RPC3
计算分子量 61 kDa
观测分子量58 kDa
GenBank蛋白编号BC002586
基因名称 POLR3C
Gene ID (NCBI) 10623
RRIDAB_3671903
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BUI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528).

实验方案

Product Specific Protocols
WB protocol for POLR3C antibody 84367-6-RRDownload protocol
IF protocol for POLR3C antibody 84367-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols