验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, NIH/3T3 cells, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27864-1-AP targets POLR3C in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | POLR3C fusion protein Ag27311 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (RNA) III (DNA directed) polypeptide C (62kD) |
| 别名 | POLR3C, RNA polymerase III subunit C3, RPC3, RPC62 |
| 计算分子量 | 61 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC002586 |
| 基因名称 | POLR3C |
| Gene ID (NCBI) | 10623 |
| RRID | AB_3669626 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BUI4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR3C antibody 27864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |