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验证数据展示

  • WB analysis using 27864-1-AP

    WB analysis using 27864-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27864-1-AP (POLR3C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 27864-1-AP

    IHC staining of mouse kidney using 27864-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27864-1-AP (POLR3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POLR3C Polyclonal antibody

POLR3C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27864-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, RPC3, RPC62

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, NIH/3T3 cells, human placenta tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27864-1-AP targets POLR3C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27311

Product name: Recombinant human POLR3C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 196-397 aa of BC002586

Sequence: IGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) III (DNA directed) polypeptide C (62kD)
别名 DNA-directed RNA polymerase III subunit C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, RPC3, RPC62
计算分子量 61 kDa
观测分子量58 kDa
GenBank蛋白编号BC002586
基因名称 POLR3C
Gene ID (NCBI) 10623
RRIDAB_3669626
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutations in the POLR3A, POLR3C, POLR3E, and POLR3F subunits are associated with susceptibility to varicella zoster virus-induced encephalitis and pneumonitis (PMID: 34395528).

实验方案

Product Specific Protocols
WB protocol for POLR3C antibody 27864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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