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验证数据展示

  • WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

    WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POLR2I antibody included
  • IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with POLR2I antibody included
  • IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

    IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

POLR2I Polyclonal antibody, PBS Only

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17270-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy POLR2I Ready-To-Use IHC Kit. POLR2I primary antibody included.

产品信息

17270-1-PBS targets POLR2I in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11027

Product name: Recombinant human POLR2I protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-125 aa of BC017112

Sequence: MEPDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSARAEDAMRLYYVCTAPHCGHRWTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
别名 DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9
计算分子量 125 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC017112
基因名称 POLR2I
Gene ID (NCBI) 5438
RRIDAB_10836936
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36954
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

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