验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A375 cells, Jurkat cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18497-1-AP targets POLR2G in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | POLR2G fusion protein Ag13338 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (RNA) II (DNA directed) polypeptide G |
| 别名 | hRPB19, hsRPB7, POLR2G, RNA polymerase II subunit B7, RPB19, RPB7 |
| 计算分子量 | 172 aa, 19 kDa |
| 观测分子量 | 20-25 kDa |
| GenBank蛋白编号 | BC112164 |
| 基因名称 | POLR2G |
| Gene ID (NCBI) | 5436 |
| RRID | AB_3085564 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62487 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POLR2G (DNA- directed RNA polymerase II subunit G), also known as hRPB19, hsRPB7 and RPB7, is the seventh largest subunit of RNA polymerase II. POLR2G functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POLR2G antibody 18497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
