验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat liver tissue, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy POLR2D Ready-To-Use IHC Kit. POLR2D primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
16093-1-AP targets POLR2D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag8701 Product name: Recombinant human POLR2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC017205 Sequence: MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (RNA) II (DNA directed) polypeptide D |
| 别名 | DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, RNA polymerase II RPB4 |
| 计算分子量 | 142 aa, 16 kDa |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | BC017205 |
| 基因名称 | POLR2D |
| Gene ID (NCBI) | 5433 |
| RRID | AB_2284144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15514 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLR2D antibody 16093-1-AP | Download protocol |
| IP protocol for POLR2D antibody 16093-1-AP | Download protocol |
| WB protocol for POLR2D antibody 16093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
Elife A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation. |
