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验证数据展示

  • WB analysis of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

    WB analysis of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

    IP experiment of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

    IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

  • WB analysis using 20595-1-AP (same clone as 20595-1-PBS)

    WB analysis using 20595-1-AP (same clone as 20595-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

POLR1A Polyclonal antibody, PBS Only

POLR1A Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 20595-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20595-1-PBS targets POLR1A in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14543

Product name: Recombinant human RPA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 295-574 aa of BC017115

Sequence: DDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (RNA) I polypeptide A, 194kDa
别名
计算分子量 1720 aa, 195 kDa
观测分子量 190-195 kDa
GenBank蛋白编号BC017115
基因名称 POLR1A
Gene ID (NCBI) 25885
RRIDAB_10949227
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95602
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

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