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验证数据展示

  • IF Staining of A375 using 28590-1-AP (same clone as 28590-1-PBS)

    IF Staining of A375 using 28590-1-AP (same clone as 28590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 28590-1-AP (same clone as 28590-1-PBS)

    IF Staining of A431 using 28590-1-AP (same clone as 28590-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

  • WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

    WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

  • WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

    WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

POLQ Polyclonal antibody, PBS Only

POLQ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28590-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28590-1-PBS targets POLQ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29643

Product name: Recombinant human POLQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2342-2590 aa of NM_199420

Sequence: LSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), theta
别名 POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta
计算分子量 290 kDa
观测分子量250~290 kDa, 197 kDa
GenBank蛋白编号NM_199420
基因名称 POLQ
Gene ID (NCBI) 10721
RRIDAB_3086067
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75417
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).

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