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验证数据展示

  • WB analysis of BxPC-3 using 28133-1-AP

    WB analysis of BxPC-3 using 28133-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 28133-1-AP (POLH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

POLH Polyclonal antibody

POLH Polyclonal Antibody for WB, ELISA
Cat No. 28133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28133-1-AP targets POLH in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 POLH fusion protein Ag27973 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), eta
别名 DNA polymerase eta, FLJ16395, FLJ21978, POLH, polymerase (DNA directed), eta, RAD30, RAD30 homolog A, RAD30A, XP V, XPV
计算分子量713aa,78 kDa; 414aa,46 kDa
观测分子量 78-85 kDa
GenBank蛋白编号BC015742
基因名称 POLH
Gene ID (NCBI) 5429
RRIDAB_2881070
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLH antibody 28133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols