×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 14110-1-AP (same clone as 14110-1-PBS)

    WB analysis using 14110-1-AP (same clone as 14110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14110-1-AP (POLG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14110-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 14110-1-AP (same clone as 14110-1-PBS)

    WB analysis of HEK-293T using 14110-1-AP (same clone as 14110-1-PBS)

    WB result of POLG antibody (14110-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLG transfected HEK-293T cells. This data was developed using the same antibody clone with 14110-1-PBS in a different storage buffer formulation.

POLG Polyclonal antibody, PBS Only

POLG Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14110-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

14110-1-PBS targets POLG in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5256

Product name: Recombinant human POLG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 888-1239 aa of BC042571

Sequence: GADVDSQELWIAAVLGDAHFAGMHGCTAFGWMTLQGRKSRGTDLHSKTATTVGISREHAKIFNYGRIYGAGQPFAERLLMQFNHRLTQQEAAEKAQQMYAATKGLRWYRLSDEGEWLVRELNLPVDRTEGGWISLQDLRKVQRETARKSQWKKWEVVAERAWKGGTESEMFNKLESIATSDIPRTPVLGCCISRALEPSAVQEEFMTSRVNWVVQSSAVDYLHLMLVAMKWLFEEFAIDGRFCISIHDEVRYLVREEDRYRAALALQITNLLTRCMFAYKLGLNDLPQSVAFFSAVDIDRCLRKEVTMDCKTPSNPTGMERRYGIPQGEALDIYQIIELTKGSLEKRSQPGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), gamma
别名 3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-
计算分子量 140 kDa
观测分子量 130-150 kDa
GenBank蛋白编号BC042571
基因名称 POLG
Gene ID (NCBI) 5428
RRIDAB_3669178
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54098
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLG is the catalytic subunit of DNA polymerase gamma solely responsible for the replication of mitochondrial DNA (mtDNA). POLG replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers, and the four deoxyribonucleoside triphosphates as substrates (PMID:11477093; 11897778; 15917273; 19837034; 9558343).

||
New chat

Able™

正在加载，请稍候...