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POLE3 Polyclonal antibody

POLE3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15278-1-AP

Print datasheet

Synonyms

Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1

验证数据展示

  • WB analysis using 15278-1-AP

    WB analysis using 15278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15278-1-AP (POLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15278-1-AP

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human liver cancer using 15278-1-AP

    IHC staining of human liver cancer using 15278-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15278-1-AP (POLE3 antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of HepG2 using 15278-1-AP

    IP experiment of HepG2 using 15278-1-AP

    IP result of anti-POLE3 (IP:15278-1-AP, 4ug; Detection:15278-1-AP 1:1000) with HepG2 cells lysate 1800ug.

  • IF Staining of HepG2 using 15278-1-AP

    IF Staining of HepG2 using 15278-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15278-1-AP (POLE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15278-1-AP targets POLE3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen POLE3 fusion protein Ag7396 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed), epsilon 3 (p17 subunit)
Synonyms Arsenic transactivated protein, AsTP, CHARAC17, CHRAC 17, CHRAC17, DNA polymerase II subunit 3, HuCHRAC17, p17, POLE3, YBL1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC003166
Gene Symbol POLE3
Gene ID (NCBI) 54107
RRIDAB_2166751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for POLE3 antibody 15278-1-APDownload protocol
IHC protocol for POLE3 antibody 15278-1-APDownload protocol
IF protocol for POLE3 antibody 15278-1-APDownload protocol
IP protocol for POLE3 antibody 15278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

SNORA42 enhances prostate cancer cell viability, migration and EMT and is correlated with prostate cancer poor prognosis.

Authors - Chuanyou Yi
View Article
humanWB

Front Oncol

ASF1B Promotes Oncogenesis in Lung Adenocarcinoma and Other Cancer Types.

Authors - Wencheng Zhang
View Article