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SKAR Polyclonal antibody

SKAR Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17466-1-AP

Print datasheet

Synonyms

KIAA1649, p46, PDIP46, POLDIP3, S6K1 Aly/REF like target, SKAR

验证数据展示

  • IP experiment of mouse brain using 17466-1-AP

    IP experiment of mouse brain using 17466-1-AP

    IP result of anti-SKAR (IP:17466-1-AP, 4ug; Detection:17466-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

  • WB analysis of HeLa using 17466-1-AP

    WB analysis of HeLa using 17466-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17466-1-AP (SKAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17466-1-AP

    IF Staining of HeLa using 17466-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17466-1-AP (SKAR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HepG2 cells, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy POLDIP3/SKAR Ready-To-Use IHC Kit. POLDIP3/SKAR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17466-1-AP targets SKAR in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SKAR fusion protein Ag11338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA-directed), delta interacting protein 3
Synonyms KIAA1649, p46, PDIP46, POLDIP3, S6K1 Aly/REF like target, SKAR
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC019643
Gene Symbol POLDIP3
Gene ID (NCBI) 84271
RRIDAB_2166620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKAR antibody 17466-1-APDownload protocol
IF protocol for SKAR antibody 17466-1-APDownload protocol
IP protocol for SKAR antibody 17466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ.

Authors - Xiaoxiao Wang
View Article
humanWB,IF

Sci Rep

TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins.

Authors - S Prpar Mihevc
View Article
humanWB

Am J Cancer Res

Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress.

Authors - Xin Zhao
View Article