验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy POLDIP3/SKAR Ready-To-Use IHC Kit. POLDIP3/SKAR primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
17466-1-AP targets SKAR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag11338 Product name: Recombinant human POLDIP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-229 aa of BC019643 Sequence: MADISLDELIRKRGAAAKGRLNARPGVGGVRSRVGIQQGLLSQSTRTATFQQRFDARQKIGLSDARLKLGVKDAREKLLQKDARFRIKGKVQDAREMLNSRKQQTTVPQKPRQVADAREKISLKRSSPAAFINPPIGTVTPALKLTKTIQNLYDLDEDDDGIASVPTKQMKFAASGGFLHHMAGLSSSKLSMSKALPLTKVVQNDAYTAPALPSSIRTKALPVLTSPGT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA-directed), delta interacting protein 3 |
| 别名 | POLDIP3, KIAA1649, p46, PDIP3, PDIP46 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC019643 |
| 基因名称 | SKAR |
| Gene ID (NCBI) | 84271 |
| RRID | AB_2166620 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BY77 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SKAR antibody 17466-1-AP | Download protocol |
| IHC protocol for SKAR antibody 17466-1-AP | Download protocol |
| IP protocol for SKAR antibody 17466-1-AP | Download protocol |
| WB protocol for SKAR antibody 17466-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget PDIP46 (DNA polymerase δ interacting protein 46) is an activating factor for human DNA polymerase δ. | ||
Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. | ||
Am J Cancer Res Inhibition of mTORC1 signaling sensitizes hepatocellular carcinoma cells to glycolytic stress. |
