验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy POLD3 Ready-To-Use IHC Kit. POLD3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
产品信息
21935-1-AP targets POLD3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16570 Product name: Recombinant human POLD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-360 aa of BC108908 Sequence: MLKDSGPLFNTDYDILKSNLQNCSKFSAIQCAAAVPRAPAESSSSSKKFEQSHLHMSSETQANNELTTNGHGPPASKQVSQQPKGIMGMFASKAAAKTQETNKETKTEAKEVTNASAAGNKAPGKGNMMSNFFGKAAMNKFKVNLDSEQAVKEEKIVEQPTVSVTEPKLATPAGLKKSSKKAEPVKVLQKEKKRGKRVALSDDETKETENMRKKRRRIKLPESDSSEDEVFPDSPGAYEAESPSPPPPPSPPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA-directed), delta 3, accessory subunit |
| 别名 | DNA polymerase delta subunit 3, KIAA0039, P66, P68, POLD3 |
| 计算分子量 | 466 aa, 51 kDa |
| 观测分子量 | 51-70 kDa |
| GenBank蛋白编号 | BC108908 |
| 基因名称 | POLD3 |
| Gene ID (NCBI) | 10714 |
| RRID | AB_2878949 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15054 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLD3 antibody 21935-1-AP | Download protocol |
| IP protocol for POLD3 antibody 21935-1-AP | Download protocol |
| WB protocol for POLD3 antibody 21935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev TRF1 uses a noncanonical function of TFIIH to promote telomere replication
| ||
J Adv Res Bavachinin Protects the Liver in NAFLD by Promoting Regeneration via Targeting PCNA | ||
Nucleic Acids Res Somatic and intergenerational G4C2 hexanucleotide repeat instability in a human C9orf72 knock-in mouse model | ||
Nucleic Acids Res Disruption of G-quadruplex dynamicity by BRCA2 abrogation instigates phase separation and break-induced replication at telomeres |
