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验证数据展示

  • IF Staining of HeLa using 86197-2-RR

    IF Staining of HeLa using 86197-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POLD1 antibody (86197-2-RR, Clone: 250828E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • WB analysis using 86197-2-RR

    WB analysis using 86197-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86197-2-RR (POLD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 86197-2-RR

    IHC staining of human colon cancer using 86197-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86197-2-RR (POLD1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86197-2-RR

    Affinity and Kinetic Characterization of 86197-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86197-2-RR against Human POLD1 were performed. The affinity constant is below 1 pM.

POLD1 Recombinant monoclonal antibody

POLD1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 86197-2-RR

产品说明书

CloneNo. 250828E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, RAW 264.7 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86197-2-RR targets POLD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8178

Product name: Recombinant human POLD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 858-1107 aa of BC008800

Sequence: EGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA directed), delta 1, catalytic subunit 125kDa
别名 3'-5' exodeoxyribonuclease, CDC2, EC:2.7.7.7, EC:3.1.11.-, POLD
计算分子量 1107 aa, 124 kDa
观测分子量 124 kDa
GenBank蛋白编号BC008800
基因名称 POLD1
Gene ID (NCBI) 5424
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP28340
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (

实验方案

Product Specific Protocols
IF protocol for POLD1 antibody 86197-2-RRDownload protocol
IHC protocol for POLD1 antibody 86197-2-RRDownload protocol
WB protocol for POLD1 antibody 86197-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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