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验证数据展示

  • WB analysis using 86680-2-RR (same clone as 86680-2-PBS)

    WB analysis using 86680-2-RR (same clone as 86680-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86680-2-RR (POLB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86680-2-PBS in a different storage buffer formulation.

  • IF Staining of COLO 320 using 86680-2-RR (same clone as 86680-2-PBS)

    IF Staining of COLO 320 using 86680-2-RR (same clone as 86680-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using POLB antibody (86680-2-RR, Clone: 251689E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86680-2-PBS in a different storage buffer formulation.

POLB Recombinant monoclonal antibody, PBS Only

POLB Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86680-2-PBS

产品说明书

CloneNo. 251689E10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, DNA polymerase beta, EC:2.7.7.7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86680-2-PBS targets POLB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12496

Product name: Recombinant human POLB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-335 aa of BC100288

Sequence: MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polymerase (DNA directed), beta
别名 5'-deoxyribose-phosphate lyase, 5'-dRP lyase, AP lyase, DNA polymerase beta, EC:2.7.7.7
计算分子量 335 aa, 38 kDa
观测分子量40 kDa
GenBank蛋白编号BC100288
基因名称 DNA Polymerase Beta
Gene ID (NCBI) 5423
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP06746
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation (PMID: 38731327).

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