验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, COLO 320 cells, HeLa cells, MCF-7 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
21778-1-AP targets POLA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0271 Product name: Recombinant human POLA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-275 aa of BC001347 Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polymerase (DNA directed), alpha 2 (70kD subunit) |
| 别名 | DNA polymerase alpha subunit B |
| 计算分子量 | 70 kDa |
| 观测分子量 | 68-70 kDa |
| GenBank蛋白编号 | BC001347 |
| 基因名称 | POLA2 |
| Gene ID (NCBI) | 23649 |
| RRID | AB_10733243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14181 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for POLA2 antibody 21778-1-AP | Download protocol |
| WB protocol for POLA2 antibody 21778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res CST does not evict elongating telomerase but prevents initiation by ssDNA binding. | ||
Cancer Cell Int Overexpression of POLA2 in hepatocellular carcinoma is involved in immune infiltration and predicts a poor prognosis | ||
Transl Cancer Res E2F-mediated POLA2 upregulation is correlated with macrophage infiltration and poor prognosis in hepatocellular carcinoma |
