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验证数据展示

  • WB analysis of A549 using 24888-1-AP

    WB analysis of A549 using 24888-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24888-1-AP (POGK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

POGK Polyclonal antibody

POGK Polyclonal Antibody for WB, ELISA
Cat No. 24888-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BASS2, KIAA1513, KIAA15131, LST003, POGK, SLTP003

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, human testis tissue, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24888-1-AP targets POGK in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21681

Product name: Recombinant human POGK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 260-609 aa of BC131624

Sequence: ALVDQRVAEYVRYMQAKGDPITREAMQLKALEIAQEMNIPEKGFKASLGWCRRMMRRYDLSLRHKVPVPQHLPEDLTEKLVTYQRSVLALRRAHDYEVAQMGNADETPICLEVPSRVTVDNQGEKPVLVKTPGREKLKITAMLGVLADGRKLPPYIILRGTYIPPGKFPSGMEIRCHRYGWMTEDLMQDWLEVVWRRRTGAVPKQRGMLILNGFRGHATDSVKNSMESMNTDMVIIPGGLTSQLQVLDVVVYKPLNDSVRAQYSNWLLAGNLALSPTGNAKKPPLGLFLEWVMVAWNSISSESIVQGFKKCHISSNLEEEDDVLWEIESELPGGGEPPKDCDTESMAESN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pogo transposable element with KRAB domain
别名 BASS2, KIAA1513, KIAA15131, LST003, POGK, SLTP003
计算分子量 609 aa, 69 kDa
观测分子量 85 kDa
GenBank蛋白编号BC131624
基因名称 POGK
Gene ID (NCBI) 57645
RRIDAB_2879779
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P215
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POGK also named as pogo transposable element with KRAB domain is a 609 amino acid protein, which contains one DDE domain, 1 KRAB domain and one HTH CENPB-type DNA binding domain. POGK belongs to DNA-mediated transposons family and may involve in the regulation of gene transcription.

实验方案

Product Specific Protocols
WB protocol for POGK antibody 24888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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