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验证数据展示

  • WB analysis using 11398-1-AP

    WB analysis using 11398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11398-1-AP (POF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 11398-1-AP

    IF Staining of MCF-7 using 11398-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11398-1-AP (POF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 11398-1-AP

    IHC staining of human ovary cancer using 11398-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 11398-1-AP

    IHC staining of human stomach cancer using 11398-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11398-1-AP (POF1B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

POF1B Polyclonal antibody

POF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11398-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein POF1B, premature ovarian failure, 1B, POF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, mouse colon tissue
Positive IHC detected inhuman ovary cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11398-1-AP targets POF1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1945

Product name: Recombinant human POF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 247-595 aa of BC017500

Sequence: DGPEKLDPRYFGELLADLSRKNTDLYHCLLEHLQRIGGSKQDFESTDESEDIESLIPKGLSEFTKQQIRYILQMRGMSDKSLRLVLSTFSNIREELGHLQNDLTSLENDKMRLEKDLSFKDTQLKEYEELLASVRANNHQQQQGLQDSSSKCQALEENNLSLRHTLSDMEYRLKELEYCKRNLEQENQNLRMQVSETCTGPMLQAKMDEIGNHYTEMVKNLRMEKDREICRLRSQLNQYHKDVSKREGSCSDFQFKLHELTSLLEEKDSLIKRQSEELSKLRQEIYSSHNQPSTGGRTTITTKKYRTQYPILGLLYDDYEYIPPGSETQTIVIEKTEDKYTCVSSLGHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 premature ovarian failure, 1B
别名 Protein POF1B, premature ovarian failure, 1B, POF
计算分子量 595 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC017500
基因名称 POF1B
Gene ID (NCBI) 79983
RRIDAB_2252552
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POF1B plays a key role in the organization of epithelial monolayers by regulating the actin cytoskeleton. POF1B is a candidate gene for premature ovarian failure (POF); it is mainly expressed in polarised epithelial tissues.

实验方案

Product Specific Protocols
IF protocol for POF1B antibody 11398-1-APDownload protocol
IHC protocol for POF1B antibody 11398-1-APDownload protocol
WB protocol for POF1B antibody 11398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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