验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22054-1-AP targets POC5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag17060 Product name: Recombinant human C5orf37 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 202-550 aa of BC101325 Sequence: RKDEVISSLSHAIGKQKEKIELMRTFFHWRIGHVRARQDVYEGKLADQYYQRTLLKKVWKVWRSVVQKQWKDVVERACQARAEEVCIQISNDYEAKVAMLSGALENAKAEIQRMQHEKEHFEDSMKKAFMRGVCALNLEAMTIFQNRNDAGIDSTNNKKEEYGPGVQGKEHSAHLDPSAPPMPLPVTSPLLPSPPAAVGGASATAVPSAASMTSTRAASASSVHVPVSALGAGSAATAASEEMYVPRVVTSAQQKAGRTITARITGRCDFASKNRISSSLAIMGVSPPMSSVVVEKHHPVTVQTIPQATAAKYPRTIHPESSTSASRSLGTRSAHTQSLTSVHSIKVVD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 5 open reading frame 37 |
| 别名 | C5orf37, Centrosomal protein POC5 |
| 计算分子量 | 575 aa, 63 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC101325 |
| 基因名称 | C5orf37 |
| Gene ID (NCBI) | 134359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NA72 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POC5 (POC5 Centriolar Protein) is a conserved protein essential for the assembly and structural integrity of the centriole, a core component of the centrosome and cilia. Localized to the distal portion of the centriole, it plays a critical role in centriole elongation, stabilization, and the maintenance of its characteristic nine-fold symmetric microtubule arrangement. By ensuring accurate centriole duplication, POC5 contributes to proper mitotic spindle formation and faithful chromosome segregation during cell division.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for POC5 antibody 22054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
