验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human brain tissue, human heart tissue, human liver tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
15552-1-AP targets PNPO in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7914 Product name: Recombinant human PNPO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC006525 Sequence: MTCWLRGVTATFGRPAEWPGYLSHLCGRSAAMDLGPMRKSYRGDREAFEETHLTSLDPVKQFAAWFEEAVQCPDIGEANAMCLATCTRDGKPSARMLLLKGFGKDGFRFFTNFESRKGKELDSNPFASLVFYWEPLNRQVRVEGPVKKLPEEEAECYFHSRPKSSQIGAVVSHQSSVIPDREYLRKKNEELEQLYQDQEVPKPKSWGGYVLYPQVMEFWQGQTNRLHDRIVFRRGLPTGDSPLGPMTHRGEEDWLYERLAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pyridoxamine 5'-phosphate oxidase |
| 别名 | Pyridoxine-5'-phosphate oxidase, Pyridoxine 5 phosphate oxidase, Pyridoxamine-phosphate oxidase, Pyridoxamine phosphate oxidase, PDXPO |
| 计算分子量 | 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC006525 |
| 基因名称 | PNPO |
| Gene ID (NCBI) | 55163 |
| RRID | AB_2165814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NVS9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PNPO antibody 15552-1-AP | Download protocol |
| IHC protocol for PNPO antibody 15552-1-AP | Download protocol |
| IP protocol for PNPO antibody 15552-1-AP | Download protocol |
| WB protocol for PNPO antibody 15552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Metab Spatial hepatocyte plasticity of gluconeogenesis during the metabolic transitions between fed, fasted and starvation states | ||
Adv Sci (Weinh) PNPO-Mediated Oxidation of DVL3 Promotes Multiple Myeloma Malignancy and Osteoclastogenesis by Activating the Wnt/β-Catenin Pathway | ||
Apoptosis Targeting PNPO to suppress tumor growth via inhibiting autophagic flux and to reverse paclitaxel resistance in ovarian cancer | ||
Nat Metab PNPO-PLP axis senses prolonged hypoxia in macrophages by regulating lysosomal activity |
