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验证数据展示

  • IHC staining of mouse cerebellum using 27787-1-AP

    IHC staining of mouse cerebellum using 27787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27787-1-AP

    IHC staining of mouse cerebellum using 27787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27787-1-AP (PNPLA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PNPLA7 Polyclonal antibody

PNPLA7 Polyclonal Antibody for IHC, ELISA
Cat No. 27787-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

RP11 48C7.2, NTEL1, NTE R1, C9orf111

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27787-1-AP targets PNPLA7 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PNPLA7 fusion protein Ag27184 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 patatin-like phospholipase domain containing 7
别名 RP11 48C7.2, NTEL1, NTE R1, C9orf111
计算分子量 146 kDa
GenBank蛋白编号NM_001098537
基因名称 PNPLA7
Gene ID (NCBI) 375775
RRIDAB_3669623
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZV29
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PNPLA7 (Patatin-like phospholipase domain-containing protein 7), also termed NTE-related 1 (NTE-R1) or NRE, is a member of the PNPLAs family, which is conserved protein in mice, rats and humans. It serves key roles in triglyceride hydrolysis, energy metabolism, lipid droplet (LD), and in regulation of adipocyte differentiation. It is reported that PNPLA7 was down-regulated in HCC through hypermethylation of its promoter, which indicates that PNPLA7 may be a potential biomarker for HCC diagnosis (PMID: 16799181, 27347198).

实验方案

Product Specific Protocols
IHC protocol for PNPLA7 antibody 27787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols