验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30905-1-AP targets PNPLA1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PNPLA1 fusion protein Ag34139 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | patatin-like phospholipase domain containing 1 |
| 别名 | PNPLA 1, ARCI10 |
| 计算分子量 | 532 aa, 58 kDa |
| 观测分子量 | 48kDa;58kDa |
| GenBank蛋白编号 | BC103907 |
| 基因名称 | PNPLA1 |
| Gene ID (NCBI) | 285848 |
| RRID | AB_3669778 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N8W4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PNPLA1 is a member of the patatin-like phospholipase (PNPLA) family. PNPLAs are highly conserved enzymes of prokaryotic and eukaryotic organisms that play an important role in lipid homeostasis. PNPLA1 has been identified as an essential transacylase for acylceramide biosynthesis to maintain the epidermal permeability barrier (PMID: 30290227). It is mainly expressed in the skin and has 3 isoforms with MW of 58, 48 and 49 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PNPLA1 antibody 30905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |