验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IP detected in | PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
13217-1-AP targets PNMT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4098 Product name: Recombinant human PNMT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC037246 Sequence: MSGADRSPNAGAAPDSAPGQAAVASAYQRFEPRAYLRNNYAPPRGDLCNPNGVGPWKLRCLAQTFATGEVSGRTLIDIGSGPTVYQLLSACSHFEDITMTDFLEVNRQELGRWLQEEPGAFNWSMYSQHACLIEGKGECWQDKERQLRARVKRVLPIDVHQPQPLGAGSPAPLPADALVSAFCLEAVSPDLASFQRALDHITTLLRPGGHLLLIGALEESWYLAGEARLTVVPVSEEEVREALVRSGYKVRDLRTYIMPAHLQTGVDDVKGVFFAWAQKVGL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phenylethanolamine N-methyltransferase |
| 别名 | PNMTase, Phenylethanolamine N-methyltransferase, PENT, Noradrenaline N-methyltransferase, EC:2.1.1.28 |
| 计算分子量 | 282 aa, 31 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC037246 |
| 基因名称 | PNMT |
| Gene ID (NCBI) | 5409 |
| RRID | AB_2167781 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11086 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phenylethanolamine N-methyltransferase(PNMT), the final enzyme in catecholamine biosynthesis, converts norepinephrine to epinephrine, thereby serving as a marker of the adrenergic phenotype.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for PNMT antibody 13217-1-AP | Download protocol |
| WB protocol for PNMT antibody 13217-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism. | ||
Front Endocrinol (Lausanne) Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension |
