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PNMT Polyclonal antibody

PNMT Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13217-1-AP

Print datasheet

Synonyms

PENT, PNMT, PNMTase

验证数据展示

  • WB analysis of K-562 using 13217-1-AP

    WB analysis of K-562 using 13217-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13217-1-AP (PNMT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-12 using 13217-1-AP

    IP experiment of PC-12 using 13217-1-AP

    IP result of anti-PNMT (IP:13217-1-AP, 4ug; Detection:13217-1-AP 1:300) with PC-12 cells lysate 2400ug.

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13217-1-AP targets PNMT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PNMT fusion protein Ag4098 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phenylethanolamine N-methyltransferase
Synonyms PENT, PNMT, PNMTase
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC037246
Gene Symbol PNMT
Gene ID (NCBI) 5409
RRIDAB_2167781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNMT antibody 13217-1-APDownload protocol
IP protocol for PNMT antibody 13217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism.

Authors - Shin Takasawa
View Article
humanWB

Front Endocrinol (Lausanne)

Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension

Authors - He Ma
View Article