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验证数据展示

  • WB analysis using 21770-1-AP (same clone as 21770-1-PBS)

    WB analysis using 21770-1-AP (same clone as 21770-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21770-1-AP (PNMA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21770-1-AP (PNMA5 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    IHC staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21770-1-AP (PNMA5 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    IF Staining of mouse testis using 21770-1-AP (same clone as 21770-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PNMA5 antibody (21770-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21770-1-PBS in a different storage buffer formulation.

PNMA5 Polyclonal antibody, PBS Only

PNMA5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 21770-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

KIAA1934, paraneoplastic antigen like 5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

21770-1-PBS targets PNMA5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16414

Product name: Recombinant human PNMA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 133-448 aa of BC101112

Sequence: SLDAEVMPQVRSPPLEPPKESMWYRKLKVFSGTASPSPGEETFEDWLEQVTEIMPIWQVSEVEKRRRLLESLRGPALSIMRVLQANNDSITVEQCLDALKQIFGDKEDFGASQFRFLQTSPKIGEKVSTFLLRLEPLLQKAVHKSPLSVRSTDMIRLKHLLARVAMTPALRGKLELLDQRGCPPNFLELMKLIRDEEEWENTEAVMKNKEKPSGRGRGASGRQARAEASVSAPQATVQARSFSDSSPQTIQGGLPPLVKRRRLLGSESTRGEDHGQATYPKAENQTPGREGPQAAGEELGNEAGAGAMSHPKPWET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 paraneoplastic antigen like 5
别名 KIAA1934, paraneoplastic antigen like 5
计算分子量 448 aa, 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC101112
基因名称 PNMA5
Gene ID (NCBI) 114824
RRIDAB_3669374
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNMA5 is a member of the paraneoplastic Ma antigen (PNMA) family proteins which share relatively conserved protein sequences and are involved in the regulation of growth, apoptosis, or fate determination in different cell types (PMID: 29378289). PNMA5 appears to be involved in the association areas of the neocortex during primate evolution (PMID: 19366867). PNMA5 has been reported to play roles in the pathological development and metastasis of some cancers, including colorectal cancer, ovarian cancer, and lung cancer (PMID: 35559417; 35117731; 34136487).

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