• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human breast cancer using 13558-1-AP

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 13558-1-AP

    IHC staining of human breast cancer using 13558-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13558-1-AP (PNKP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 13558-1-AP

    WB analysis of Jurkat using 13558-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13558-1-AP (PNKP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PNKP Polyclonal antibody

PNKP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DNA 5 kinase/3 phosphatase, PNK, PNKP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13558-1-AP targets PNKP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4421

Product name: Recombinant human PNKP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 171-521 aa of BC033822

Sequence: DLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polynucleotide kinase 3'-phosphatase
别名 DNA 5 kinase/3 phosphatase, PNK, PNKP
计算分子量 521 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC033822
基因名称 PNKP
Gene ID (NCBI) 11284
RRIDAB_2877958
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T60
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PNKP antibody 13558-1-APDownload protocol
IHC protocol for PNKP antibody 13558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols