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  • KD/KO Validated

PMPCB Polyclonal antibody

PMPCB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16064-1-AP

Print datasheet

Synonyms

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB



经过测试的应用

Positive WB detected inMCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16064-1-AP targets PMPCB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PMPCB fusion protein Ag8937 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidase (mitochondrial processing) beta
Synonyms Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB
Calculated Molecular Weight 489 aa, 54 kDa
Observed Molecular Weight 43-52 kDa
GenBank Accession NumberBC010398
Gene Symbol PMPCB
Gene ID (NCBI) 9512
RRIDAB_2167122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

实验方案

Product Specific Protocols
WB protocol for PMPCB antibody 16064-1-APDownload protocol
IHC protocol for PMPCB antibody 16064-1-APDownload protocol
IF protocol for PMPCB antibody 16064-1-APDownload protocol
IP protocol for PMPCB antibody 16064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Authors - Xiaoyan Guo
humanWB

Nat Cell Biol

PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.

Authors - Shotaro Saita
humanWB

Nat Commun

DELE1 tracks perturbed protein import and processing in human mitochondria.

Authors - Evelyn Fessler
humanWB

EMBO J

PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.

Authors - Shotaro Saita
WB,IF

Autophagy

The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.

Authors - Giulia Bertolin
  • KD Validated
humanWB

Cell Rep

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Authors - Fenglei Jian