• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 26536-1-AP

    WB analysis using 26536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26536-1-AP (PMPCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of T-47D using 26536-1-AP

    IF Staining of T-47D using 26536-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 26536-1-AP (PMPCA antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of MDA-MB-453s using 26536-1-AP

    IP experiment of MDA-MB-453s using 26536-1-AP

    IP result of anti-PMPCA (IP:26536-1-AP, 4ug; Detection:26536-1-AP 1:500) with MDA-MB-453s cells lysate 2800ug.

PMPCA Polyclonal antibody

PMPCA Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 26536-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMDA-MB-453s cells, T-47D cells
Positive IP detected inMDA-MB-453s cells
Positive IF/ICC detected inT-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26536-1-AP targets PMPCA in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag19206

Product name: Recombinant human PMPCA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 176-525 aa of BC132724

Sequence: LTDEEVEMTRMAVQFELEDLNLRPDPEPLLTEMIHEAAYRENTVGLHRFCPTENVAKINREVLHSYLRNYYTPDRMVLAGVGVEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNVSLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGGTVDTVELERAKTQLTSMLMMNLESRPVIFEDVGRQVLATRSRKLPHELCTLIRNVKPEDVKRVASKMLRGKPAVAALGDLTDLPTYEHIQTALSSKDGRLPRTYRLFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidase (mitochondrial processing) alpha
别名 Alpha MPP, INPP5E, KIAA0123, MPPA, P 55, PMPCA
计算分子量 525 aa, 58 kDa
观测分子量 50-58 kDa
GenBank蛋白编号BC132724
基因名称 PMPCA
Gene ID (NCBI) 23203
RRIDAB_2880545
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10713
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PMPCA antibody 26536-1-APDownload protocol
IP protocol for PMPCA antibody 26536-1-APDownload protocol
WB protocol for PMPCA antibody 26536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Enhanced mapping of small-molecule binding sites in cells

Authors - Jacob M Wozniak
View Article
humanWB

Hum Mol Genet

GRP75 overexpression rescues frataxin deficiency and mitochondrial phenotypes in Friedreich Ataxia cellular models.

Authors - Yi Na Dong
View Article
||
New chat Able
正在加载，请稍候...